Showing Visitor Messages 1 to 1 of 1
-
Chao cac ban,
Minh moi su dung may sac ky nen chua co nhieu kinh nghiem.
Hien nay minh dang muon tach mot so nucleotids. Minh dang chay gradient, pha dong gom dem KH2PO4 va ACN.
Nhung co 2 nucleotid trung thoi gian luu, o tach duoc.
Ban nao co kinh nghiem ve tach sac ky cac nucleotid, chi cho minh kinh nghiem nhe.
Minh thay nguoi ta dung tetra n-butyl ammonium hydrogen sulfate (TBAS) de chay sac ky cap ion ( ion-pair). Minh van chua hieu ve co che nay. Minh co the o chay kieu sac ky nay dc khong? Hoac thay TBAS bang 1 chat khac dc o? Luc do, pha dong se nhu the nao?
